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Andre Senecal

Andre Senecal

U. S. Army NSRDEC, USA

Title: Bacteriophages: A novel technology for improving safety of produce

Biography

Biography: Andre Senecal

Abstract

The incidence of foodborne outbreaks involving fresh produce is of worldwide concern. The United States is deployed worldwide to places where lack food sanitation standards food safety enforcement could result in food-borne disease outbreaks. These outbreaks have potential harmful effects on troop health and readiness. Simultaneously, military commanders want to maximize the availability of freshly prepared meals, to improve the overall troop morale. Fruits and vegetables to support these meals are generally procured locally at the region of deployment. Fresh produce are commonly consumed uncooked and have been identified as primary causes of illnesses associated with outbreaks of foodborne disease. Therefore, strategies to eliminate food pathogens on fresh produce must be designed and validated for military and civilian use. An old anti-pathogen technology that has emerged as a novel method for improving produce safety is bacteriophages. Bacteriophages are naturally occurring predators of bacteria that can reduce the levels of their specifically-targeted pathogenic bacteria. The Army has worked with industry to develop a series of lytic bacteriophage cocktails specific against Escherichia coli O157:H7, Salmonella and Shigella. Studies were conducted to identify potential best approaches for eliminating these pathogens from broccoli, cantaloupe and strawberries. Bacteriophage cocktails, levulinic acid produce wash, and a combination of both treatments (BCPW) was compared to the Army standard of washing produce in 200 ppm free available chlorine. Our findings indicated that the BCPW treatment was a very effective method for treating produce contaminated with these pathogens even in the presence of elevated organic loads.

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